Bruce Livett's Recent Research Publications 1998-2009

Publications in Refereed Journals

1. REVIEW ARTICLES

Livett, B.G. (2009) Chromaffin cells: model cells for neuronal cell biology. Encyclopedia of Neuroscience, 3rd Edition. Edited by George Adelman and Barry H. Smith, Elsevier, Amsterdam. (Invited Review) available on CD-ROM, ISBN: 0444514325

Livett, B.G., Sandall, D.W., Keays, D., Down, J., Gayler, K.R., Satkunanathan, N. and Khalil, Z. (2006) Therapeutic applications of conotoxins that target the neuronal nicotinic acetylcholine receptor. Toxicon 48: 810–829 [Abstract] [Article pdf].

Livett, B.G., Gayler, K.R. and Khalil, Z. (2004) Drugs from the sea - Conopeptides as potential therapeutics. Current Medicinal Chemistry. (Hot Topic Issue on Marine Natural Products), Editor, Vassilios Roussis. Bentham Science Publishers, Current Medicinal Chemistry 11: 1715-1723. [Abstract] [Article pdf].

Gayler, K., Sandall, D., Greening, D., Keays, D., Polidano, M., Livett B., Down J., Satkunanathan N. and Khalil, Z. (2000) Molecular Prospecting for Drugs from the Sea. IEEE Engineering in Medicine and Biology Magazine. 24(2)80-84. [Abstract] [Article pdf].

Marley, P.D., and Livett, B.G. (2004) (INVITED REVIEW) "Chromaffin Cells: Model Cells for Neuronal Cell Biology", in "Encyclopedia of Neuroscience" 3rd. Ed., Revised and Enlarged. Eds. George Adelman and Barry H. Smith. ISBN 0444514325 ; CD-ROM - ISBN: 0120442663, Elsevier Science Publishers, BV., Amsterdam

Alonso, D., Khalil, Z., Satkunanathan, N. and Livett, B.G. (2003) Drugs from the sea:Conotoxins as drug leads for neuropathic pain and other neurological conditions. Mini Reviews in Medicinal Chemistry, 3: 785 - 787.[Abstract] [Article pdf].

Livett, B.G. and Marley, P.D. (1999) Chromaffin Cells and Chromaffin Granules, in "Encyclopedia of Neuroscience" 2nd. Ed., Revised and Enlarged., pp. 388-392. Also in CD-ROM (version 1.1), ISBN: 0-444-50160-6, Elsevier Science Publishers, BV. Amsterdam.

2. SCIENTIFIC RESEARCH PAPERS

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Townsend, A., Livett, BG, Bingham, J-P, Truong, H-T, Karas, JA, O’Donnell, P, Williamson, NA, Purcell, AW and Scanlon D (2009) Mass spectral identification of Vc1.1 and differential distribution of conopeptides in the venom duct of Conus victoriae. Effect of post-translational modifications and disulfide isomerisation on bioactivity. Int. J. Peptide Res and Therap. 15 (3): 195-203. [Abstract], [ Article.pdf]

MacRaild CA, Illesinghe J, van Lierop BJ, Townsend AL, Chebib M, Livett BG, Robinson AJ, Norton RS (2009) Structure and activity of (2,8)-dicarba-(3,12)-cystino alpha-ImI, an alpha-conotoxin containing a nonreducible cystine analogue. J Med Chem. 52 : 755-762. [Abstract], [ Article.pdf], [ SUPPORTING INFO.pdf]

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Satkunanathan, N., Livett, B., Gayler, K., Sandall, D., Down, J. and Khalil, Z. (2005) Alpha-conotoxin Vc1.1, a neuronal nicotinic receptor antagonist, alleviates neuropathic pain and accelerates functional recovery of injured neurones. Brain Res. 1059:149-158.[Abstract] [Article pdf].

Bingham, J-P., Broxton, N.M., Livett, B.G., Down, J.G., Jones, A. and Mocdlowski, E. G.(2005) Optimizing the connectivity in disulfide-rich peptides: a-conotoxin SII as a case study. Analytical Biochemistry. 338: 48-61. [Abstract] [Article pdf].

Jakubowski, J.A., Keays, D.A., Kelley, W.P., Sandall, D.W., Bingham, J-P., Livett, B.G., Gayler, K.R. and Sweedler, J.V. (2004) Determining sequences and post-translational modifications of novel conotoxins in Conus victoriae using cDNA sequencing and mass spectrometry. J. Mass Spectrometry 39: 548-557. [Abstract] [Article pdf].

Livett, B.G. and Borges, R. (2004) Advances in cell culture for chromaffin cells and related cell types - Workshop. In: Cell Biology of the Chromaffin Cell 2004. (Ricardo Borges and Louis Gandia, Editors), Fundacion Teofilo Hernando, Madrid, © Instituto Teófilo Hernando. Gabinete de publicaciones. La Laguna and Madrid. (Spain). 2004. pp. 261-267. ISBN. 84-688-7007-2
Also available electronically at http://webpages.ull.es/users/isccb12/PDF-Final/39-Livett.pdf

Fodero, L.R., Mok, S. S., Losic, D., Martin, L. L., Aguilar, M. I., Barrow, C. J., Livett, B. G. and Small, D. H. (2004) alpha7-Nicotinic acetylcholine receptors mediate an Abeta 1.42 -induced increase in the level of acetylcholinesterase in primary cortical neurons. J. Neurochem. 88: 1186-1193. [Abstract] [Article pdf].

Duda, T.F. Jr., Bingham, J-P., Livett, B.G., Kohn, A.J., Raybaudi Massilia, G., Schultz, J.R., Down, J., Sandall, D. and Sweedler, J.V. (2004) How Much at Risk Are Cone Snails? Science 303: 955-957. [Abstract] [Article pdf].

Sandall, D.W., Satkunanathan, N., Keays, D.A., Polidano, M. A., Liping, X., Pham, V., Down, J.G, Khalil, Z., Livett, B.G. and Gayler, K.R. (2003) A novel alpha-conotoxin identified by gene sequencing is active in suppressing the vascular response to selective stimulation of sensory nerves in vivo. Biochemistry 42: 6904-6911. [Abstract] [Article pdf].

PUBLISHED PATENT APPLICATION
Livett, B., Khalil, Z., Gayler, K., Down, J., Sandall, D. and Keays, D. (2002) Alpha conotoxin peptides with analgesic properties. WIPO PCT #WO 02/079236 A1, Published 10 October, 2002. Also as International Patent PCT/AU03/00411, 20th November 2003.
(This international application covers ACV1 specifically as a product together with protection for analogues).

Khalil, Z., Merhi, M. and Livett, B.G. (2001) Differential involvement of conotoxin-sensitive mechanisms in neurogenic vasodilatation responses: effects of age. J. Gerontol. A Biol. Sci. Med. Sci. 56: B356-B363 [abstract] [Full Text] [Adobe pdf]

Broxton, N., Miranda, L., Gehrmann, J., Down, J., Alewood, P. and Livett, B. (2000) Leu10 of a-conotoxin PnIB confers potency for neuronal nicotinic responses in bovine chromaffin cells. Eur J Pharmacol. 390: 229-236. [abstract]

Broxton, N.M., Down, J.G., Gehrmann, J., Alewood, P.F. Satchell, D.G, and Livett, B.G. (1999) a-Conotoxin ImI inhibits the a-bungarotoxin-resistant nicotinic response in bovine adrenal chromaffin cells. J. Neurochem. 72: 1656-1662. [abstract]

Loughnan, M., Bond, T., Atkins, A., Cuevas, J., Adams, D.J., Broxton, N.M., Livett, B.G., Down, J.G., Jones, A., Alewood, P.F. and Lewis, R.J. (1998) a-Conotoxin EpI, a novel sulfated peptide from Conus episcopatus that selectively targets neuronal nicotinic acetylcholine receptors. J Biol Chem. 273: 15667-15674. [abstract]


[PUBLICATIONS by other investigators relating to ACV1 - conotoxin Vc1.1]

Stix, G. (2005) A Toxin against Pain Scientific American. (April 2005), 70-75.

Lang PM, Burgstahler R, Haberberger RV, Sippel W, Grafe P. (2005) A Conus peptide blocks nicotinic receptors of unmyelinated axons in human nerves. Neuroreport. 16: 479-483.

Jakubowski, J.A. and Sweedler, J.V. (2004) Sequencing and mass profiling highly modified conotoxins using global reduction/alkylation followed by mass spectrometry. Anal Chem. 76: 6541-6547.

Jakubowski JA, Kelley WP, Sweedler JV. (2006) Screening for post-translational modifications in conotoxins using liquid chromatography/mass spectrometry: an important component of conotoxin discovery. Toxicon. 47:688-699.

Clark RJ, Fischer H, Nevin ST, Adams DJ, Craik DJ. (2006) The synthesis, structural characterization, and receptor specificity of the alpha-conotoxin Vc1.1. J Biol Chem. 281: 23254-23263.

Craik D. (2006) Animal, plant and microbial toxins - 15th World Congress. IDrugs. 2006 Oct;9(10): 679-681. No abstract available. For Meeting Report Click here.

Vincler M, Wittenauer S, Parker R, Ellison M, Olivera BM, McIntosh JM. (2006) Molecular mechanism for analgesia involving specific antagonism of alpha9alpha10 nicotinic acetylcholine receptors. Proc Natl Acad Sci U S A. 103(47):17880-17884.


2. REPORTS: Abstracts on conotoxins at scientific meetings 1999-2005

Rothacker, J., Broxton, N., Bunnage, A., Gayler, K. and Livett, B.G. (1999) Application of 'RACE' technology to the identification of putative conopeptides. Proc. 24th Annual Conference on Protein Structure and Function, 7-11 February, Lorne, Australia, 24: B40.

Livett, B.G., Broxton, N.M., Rothacker, J.A. and Down, J.G. (1999) Functional studies with a-conotoxins that target the neuronal type nicotinic receptor in chromaffin cells. Proc. 10th Int. Symposium on Chromaffin Cell Biology. August 22-27, 1999., Bergen, Norway, Symposium S VIII-4, p.112

Broxton, N., Miranda, L., Gehrmann, J., Down, J., Alewood, P. and Livett, B. G. (1999) Leu10 of a-conotoxin PnIB confers potency for the mammalian neuronal nicotinic response. Toxicon 37: 1460.

Down, J. G. (1999) Sequence motifs among a-conotoxins that target the muscle-type or neuronal-type nicotinic receptors. Toxicon 37: 1463.

Livett, B.G., Rothacker, J.A., Broxton, N. and Rolls, K. (1999) Real-time video capture of envenomation of prey by Conus textile, Conus marmoreus, and Conus magus. Toxicon 37: 1469-1471.

Loughnan, M., Gehrmann, J., Hu, S.H., Bond, T., Atkins, A., Jones, A., Cuevas, J., Adams, D.J., Broxton, N.M., Livett, B.G., Down, J.G., Martin, J., Craik, D.C., Alewood, P.F. and Lewis R. J. (1999) a-Conotoxin EpI and some functional and structural comparisons among related nAChR inhibitors. Toxicon 37: 1471-1472.

Peisley, A., Livett, B.G., Capon, R.J. and Down, J.G. (1999) Development of a rapid chemiluminescence detection technique for high-throughput screening of biologically active samples. Toxicon 37: 1479 - 1480.

Rothacker, J., Broxton, N., Bunnage, A., Gayler, K. and Livett, B.G. (1999) Application of 'race' technology to the identification of putative conopeptides. Toxicon 37: 1482-1483.

Livett, B.G., Broxton, N.M. and Down, J.G. (1999) Functional studies with a-conotoxins that target the neuronal-type nicotinic receptor. Toxicon 37:

Livett, B.G. and Adams, D. (2000) The neuronal nicotinic receptor (Symposium 4-1). Proc. Aust.Neuroscience. Soc. 11: 14.

Livett, B.G., Broxton, N.M., Rothacker, J.A., Sandall, D., Muscat, A., Gayler, K.R. and Down, J.G. (2000) Pharmacological discrimination of neuronal nicotinic receptors. Proc. Aust.Neuroscience. Soc. 11: 15

Polidano, M., Broxton, N. and Livett, B.G. (2000) Unmasking nicotinic receptor subtypes in bovine chromaffin cells. Proc. Aust.Neuroscience. Soc. 11: 161.

Down, J.G. and Livett, B.G. (2000) Sequence motifs among a-conotoxins which discriminate between muscle- and neuronal-type nicotinic receptors. Proc. Aust.Neuroscience. Soc. 11: 233.

Broxton, N.M., Rothacker, J.R., Sandall, D.W., Down, J.G., Gayler, K.R. and Livett, B.G. (2000) A novel a-conotoxin, identified by gene probing, is present in a number of different Conus species. Proc. Aust.Neuroscience. Soc. 11: 249.

Sandall, D., Rothacker, J., Broxton, N., Muscat, A., Down, J., Gayler, K. and Livett, B. (2000) Novel conotoxin gene products inhibit the neuronal nicotinic response. Proc. Aust.Neuroscience. Soc. 11: 177.

Rothacker, J., Catimel, B., Livett, B. and Nice, E. (2000) An investigation of surface viability in cell based biosensor studies. Proc. 25th Annual Conference on Protein Structure and Function, 6-10 February, Lorne, Australia, 25: B40.

Polidano, M., Horton, D., Lewis, R., Alewood, P., Livett, B. and Wonnacott, S. (2000) a-Conotoxins exhibit subtype selectivity for the neuronal nicotinic receptor. 1st Lilly Neuronal Nicotinic Receptors Symposium. Egham, Surrey, UK September 26-27, 2000. Molecular Pharmacology Session, Abstract #7.

Livett, B., Rothacker, J., Broxton, N., Rolls, K. and Paul, D. (2000) Video observations in real time capture envenomation of prey by Conus textile, Conus marmoreus and Conus magus. Molluscs 2000 : Understanding Molluscan Biodiversity in our Region into the 21st Century. University of Sydney, Sydney, NSW. Australia 4-8 December 2000, 54-55.

Polidano, M., Horton, D., Lewis, R., Alewood, P., Livett, B. and Wonnacott, S. (2001) a-Conotoxins exhibit subtype selectivity for the neuronal nicotinic receptor. Proc. 26th Annual Lorne Conference on Protein Structure and Function, 4-8 February, Lorne, Australia, 26: A53.

Sandall, D., Keays, D., Down, J., Bingham, J., Livett, B. and Gayler, K. (2001) Conotoxin diversity in venom from Australian Cone shells. Proc. 26th Annual Lorne Conference on Protein Structure and Function, 4-8 February, Lorne, Australia, 26: A56.

Sandall, D., Keays, D., Down, J., Livett, B. and Gayler, K. (2001) Conus peptides and the rapidly diverging gene families ; giving the snail its sting. In: gene and Genome Duplications and the Evolution of Novel Gene Functions", Aussios, France, 26-30 April, 2001. pp. xx.

Van Damme, M-P., Gould, J., Livett, B., Macaulay, J., Maxwell, R. and Parslow, G. (2001) Metabolic challenges on CD-ROMs. Proceedings of the AMEE (Association for Medical Education in Europe) conference on "Medical Education and Standards at a Time of Change", Berlin, 2-5 September, page 4.84.

Livett, B.G. (2001) Interview for Calliostoma (to be reprinted by Conchologists of America, COA, Newsletter) in a series of interviews with world malacologists. 6pp. (Interviewed by Eudardo Moreira). Published in American Conchologist Februrary 2002, p. 2 and 7.

Polidano, M.A. and Livett, B.G. (2002) 3H-Epibatidine binding to bovine adrenal membranes reveals two pharmacologically distinct neuronal nicotinic receptor subtypes. Proc. Aust. Neuroscience. Soc 13: 199.

Sandall, D.W., Satkunanathan, N., Bingham, J-P., Moczydlowski, E., Down, J.G., Livett, B.G. and Gayler, K.R. (2002) ?-Conotoxins from Conus anemone. Proc. 6th Asia-Pacific Congress on Animal, Plant and Microbial Toxins. Int. Soc. Toxinology, Symposium 19, Paper 2, Cairns, July 8-12, 2002, p. 85.

Satkunanathan, N., Khalil, Z., Sandall, D.W., Polidano, M., Keays, D., Down, J., Gayler, K.R. and Livett, B.G. (2002) An ?-conotoxin from an Australian Conus is a potent analgesic in a rat model of neuropathic pain. Venoms to Drugs 2002, Heron Is. Queensland, July 14-19, 2002, p. 30.

Sandall, D.W., O’Donnell, Leiping, X., Satkunanathan, N., Livett, B.G., Down, J.G., and Gayler, K.R. (2002) Characterization of an ?-conotoxin from Conus virgo. Venoms to Drugs 2002, July 14-19, 2002, p. 44.

Livett, B.G., Sandall, D.W., O’Donnell, Leiping, X., Satkunanathan, N and Gayler, K.R. (2002) Biochemical and pharmacological characterization of an ?-conotoxin from Conus virgo. ComBio 2002, September 29 - October 4, Sydney, POS-TUE-130.

Van Damme, M-P., Gould, J., Macaulay, J., Maxwell, R., Livett, B. and Parslow, G. (2003) Metabolic challenges on CD-ROMs. Proc. IUBMB meeting Satellite Conference on Teaching, Toronto, Canada July 2-9, 2003.

Livett, B., Satkunanathan, N., Khalil, Z., Sandall, D., Keays, D., Down, J. and Gayler, K. (2003) a-Conotoxin Vc1.1 from Conus victoriae is a potent analgesic in a rat model of human neuropathic pain. Troisieme Congres International des Societes Europeennes de Malacologie , La Rochelle, France, June 24-27, 2003.

Polidano, M., Marley, P.. and Livett, B. (2003) [3H]-Epibatidine labels two a3-containing subtypes in bovine adrenal medullary membranes. 12TH ISCCB Meeting, La Palma, Spain, 20-25 September, 2003

Livett, B.G. and Borges, R. (2003) Advances in cell culture of chromaffin cells and related cell types. Workshop, 12TH ISCCB Meeting, La Palma, Spain, 19-25 September, 2003

Khalil, Z., Satkunanathan, N., Gayler, K. and Livett, B. (2004) Striking the balance between inflammation and pain to improve neuronal repair. ANS Satellite meeting: “Understanding Pain - Biology to Behaviour”, Melbourne, Australia, January 27, 2004.

Livett, B.G. (2005) The primary afferent neuron and pain mechanisms: the onus is on Conus. Sense and Sensibility. A Symposium to honour Dr. Loris Chahl. The University of Newcastle, School of Biological Sciences. May 25, 2005.

Satkunanathan, N., Livett, B., Gayler, K. and Khalil, Z. (2005) Alpha conotoxin Vc1.1 suppresses pain and accelerates functional recovery of injured neurons. 11th World Congress on Pain. Sydney, Australia. August 21-26, 2005. Proc. Int. Assoc. for the Study of Pain (IASP). xxx – xxx

McIntosh, D., Khalil, Z., Livett, B., Gayler, K., Sandall, D., Keays, D., Down, J., Satkunanathan, N., McCracken, A., Belyea, C., Smith, M. and Wyse, B. (2005) ACV1, a novel alpha-conotoxin, promotes functional recovery in injured neurons and reduces oxidative stress levels.  Proceedings Venoms to Drugs 3, (28 Aug-2 Sept, 2005), Heron Is. Queensland, Australia (ORAL) Invited symposium speaker.

McIntosh, D, Khalil, Z, Satkunanathan, N, McCracken A, Belyea, C, Sandall, D.K, Keays, D.A., Down, J.G., Gayler, K,R and Livett, B.G. (2005) Toxicological studies on ACV1, an {alpha}-conopeptide from Conus victoriae with analgesic properties.  Proceedings Venoms to Drugs 3, (28 Aug-2 Sept, 2005), Heron Is. Queensland, Australia. (POSTER)

Khalil, Z., Livett, B., Gayler, K., Sandall, D., Keays, D., Down, J., Satkunanathan, N., McIntosh, D., Mc Cracken, A. and Belyea, C. (2005) ACV1, a novel alpha-conotoxin, promotes functional recovery in injured neurons and reduces oxidative stress levels. Proc. XVIIIth World Congress of Neurology, 5-11 November 2005. WCN2005-1260.

Livett, B.G., (2006) Conotoxins as analgesics: Is the new kid on the block finally shaping up ? Topical Workshop, 26th Annual Scientific Meeting of the Australian Pain Society. 9-12 April, 2006, Grand Hyatt Hotel, Melbourne. (Invited speaker and Chair of this Topical Workshop)

Townsend, A., Scanlon, D., ODonnell, P., Inserra, M., Bingham, J-P., Satkunanathan, N., Khalil Z., Purcell, A. and Livett, B.G. (2006) Posttranslational  modifications of venom components in Conus victoriae. Proc. 15th World Congress on Animal, Plant and Microbial Toxins, Glasgow (July 23-28), 2006, p. 280.

Satkunanathan, N., Khodr, B., Georgiou, G., McIntosh, D., Belyea, C., Khalil, Z. and Livett, B.G. (2006) An alpha-conotoxin from Conus victoriae is effective at alleviating neuropathic pain in an animal model of diabetic neuropathy. Proc. 15th World Congress on Animal, Plant and Microbial Toxins, Glasgow (July 23-28), 2006, p. 330

Townsend, A, Scanlon, D, ODonnell, P, Inserra, M, Bingham, J-P, Satkunanathan, N, Khalil Z, Purcell, A. and Livett, B.G. (2006) Conotoxin Vc1.1 (ACV1) is present as posttranslationally modified and dimeric forms in duct venom of the marine cone snail, Conus victoriae. Australian Chemical Institute Conference 'Drug Design Amongst the Vines', Hunter Valley, 3-7 December 2006. (ORAL) Abstract #49

Satkunanathan, N, Khodr, B, Georgiou, G, McIntosh, D, Belyea, C, Khalil Z. and Livett, B.G. (2006) ACV1, an alpha-conotoxin from Conus victoriae alleviates  neuropathic pain in an animal model of diabetic neuropathy. Australian Chemical Institute Conference 'Drug Design Amongst the Vines', Hunter Valley, 3-7 December 2006. (POSTER) Abstract #60

Updated 20 December, 2006

Reports (in-house reports to the University)

Livett, B.G., Maxwell, R. and Macauley, J. (1999) Nutritional aspects of diabetes: A Melbourne/Monash perspective. DITAM'99 Showcase of Projects, November 1999,